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Although fundamental 5A-1MQ and LD-mediated changes to the microbiome were identified, this work is similar to many current studies where further investigations will clarify if changes in the microbiome are causative or associative 100

Culturing was performed in RPMI 1640 medium enriched with 10% fetal bovine serum, maintained at 37C in a humidified incubator with an atmosphere of 5% CO 2 and 95% air